The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA. Rapidly detect as little as 0. Microplate reader setup for a Quant-iT 1X dsDNA HS Assay. Qubit assays are available for double- and single-stranded DNA (dsDNA and ssDNA), RNA, microRNA, and protein. Three kits covering a wide range of dsDNA concentrations. Calibrator MPO. 0 fluorometer (Selection Guide for the Qubit and Quant-iT Assay Kits. 2. Figure 2. 3. Quant-iT™ PicoGreen® dsDNA Assay Kit *10 x 100 µl* 1 kit P11496 Quant-iT™ PicoGreen® dsDNA Reagent 1 kit P7581 Quant-iT™ PicoGreen® dsDNA Reagent *10 x 100 µl* 1 kit P11495 Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 1 kit R11491 Quant-iT™ RiboGreen® RNA Assay Kit *2,000 assays* 1 kit R11490 Quant-iT™ OliGreen™ ssDNA. You will need 100 μl diluted PicoGreen reagent per well. 2 – 100 ng. In addition, the assay failed to detect in vitro generated MPO-DNA complexes when spiked into plasma. 710 citations – Kit [P7589] Quant-iT™ PicoGreen™ dsDNA Assay Kit by Thermo Scientific. Figure 2. Within two years of follow-up, the primary endpoint defined as nonfatal myocardial infarction, stroke or transient ischemic attack, cardiovascular death, and >80% target vessel restenosis occurred in 34 patients (43%). This protocol is a simplified and This protocol is a simplified and condensed version of the full protocol from the manufacturer. Qubit® dsDNA BR Assay Kit. It must be completely liquified before use by allowing it to come to room temperature. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™The Quant-iT™ dsDNA High-Sensitivity Assay Kit makes DNA quantification easy and accurate. Selectively detect dsDNA from a wide variety of sources, and even in presence of. Note that the assay volume is dependent on the instrument used to measure fl uorescence; with a microplate reader and a 96-well microplate, the assay volume is reduced to 200 μL and 2,000 assays are possible. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. The DeNovix dsDNA Broad Range Assay is ideal for measuring sample concentrations ranging from 0. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. The Quant-iT™ PicoGreen® dsDNA Assay Kit makes for an easy, reliable, and accurate method to quantify dsDNA samples when used in conjunction with the Modulus™ Microplate Fluorometer from Turner BioSystems, Inc. 5) prepared from 20x. UV흡광 결과보다 민감도 우수. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. If you would like to use this kit with the Qubit® fluorometer, we have includedQuant-iT ™ PicoGreen ™ dsDNA Reagent 1 mL reagent 10 x 100 µL P7581 P11495 TE Buffer (20X), RNase-free 100 mL T11493. Picogreen assays are compatible with most microplate readers and fluorometers. 50 pg to 2 µg. PCR products were quantified using the Quant-iT PicoGreen dsDNA Assay Kit on a Microplate reader (Bio-Tek, FLx800), and then mixed according to the amount of data required for each sample. Quant-iT ™ 1X dsDNA Assay Kit, High Sensitivity 200 pg−100 ng 1,000 reactions Q33232 Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267 Quant-iT ™ DNA Assay Kit, High Sensitivity 200 pg−100 ng 1,000 reactions Q33120 Quant-iT ™ DNA Assay Kit, Broad-Range 4 ng−1 μg 1,000 reactions Q33130 Quant-iT ™The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. Picogreen assays are compatible with most microplate readers and fluorometers. The Quantus™ NGS Starter package is cost-effective and easy to incorporate into your laboratory. samples as well as standard in the assay itself. 95 mL TE for the . DNA standards ranging from 1000 ng/mL to 50 pg/mL were assayed using the Quant-iT PicoGreen dsDNA Assay and SpectraMax M5 microplate reader. Rapidly detect as little as 0. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. • Specific for dsDNA in the presence of RNA. 5 ng/ml ~ 1,000 ng/ml 까지 정비례. 5). Rapidly detect as little as 0. • Suitable for use in 96- and 384-well plate formats. • Suitable for use in 96- and 384-well plate formats. Compared with the aforementioned methods, the PicoGreen dye binding assay for dsDNA has greater sensitivity, specificity, and linear range. Rapidly detect as little as 0. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. • Specific for dsDNA in the presence of RNA. HepG2 cell number standard curve determined using the PicoGreen® assay (Quant-iTTM PicoGreen® dsDNA reagent kit, Invitrogen), HepG2 cells were harvested from conventional 2D T75 culture flasks and counted using a haemocytometer and Trypan Blue dye exclusion. 10 x 100 µL. このアッセイは、従来のUV吸光度法に比べて感度が何倍も高いため、様々な濃度範囲に対して最適化することが可能です。. PicoGreen® Assay Supplies Equipment: − NanoDrop 3300 Fluorospectrometer − 2uL pipettor (low retention nuclease free pipette tips) Materials:Total DNA recovery was determined with the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Carlsbad, CA). DNA standard curve. The Qubit dsDNA HS and BR Assay Kits are accurate from 10 pg/L to 100 ng/uL, and 100 pg/L to 1,000 ng/L, respectively. The DeNovix dsDNA High. 作成者 LatB Staff 01. Abstract. BR reagent (Component A) 1. Picogreen assays are compatible with most microplate readers and fluorometers. 6 Add equivalent volume (100 μl) of diluted PicoGreen to every well (keeping plate in the dark as much as possible). 95) in the range of 10 pg till 100 ng for the salmon dsDNA as well as for the control dsDNA from the kit (λ dsDNA standard) (Fig. 10. , to prepare enough working solution to assay 100 samples in 200 µL volumes, add 50 µL Quant-iT ™ RiboGreen RNA Reagent to 9. Mix PicoGreen and DNA by pippetting up and down 5-10 times. The assay is highly selective for double- stranded DNA over RNA, and in the. Fluorometric dsDNA Assay. DNA. g. Qubit Flex NGS Starter Kit 1 instrument kit A45924 Microplate assays and instruments Quant-iT PicoGreen dsDNA Assay Kit 1,000 assays P11496 Quant-iT dsDNA Assay Kit, high sensitivity 1,000 assays Q33120 Quant-iT dsDNA Assay Kit, broad range 1,000 assays Q33130 Quant-iT RiboGreen RNA Assay Kit 1,000 assays R11490Quant-iT™ PicoGreen ® dsDNA Kit (Invitrogen, #P11496). Fluorescence was measured at 485/530 nm and plotted versus the mass of nucleic acid for the DNA alone,. A Qubit dsDNA BR (broad range, 2 to 1000 ng) Assay Kit and Qubit dsDNA HS (high sensitivity, 0. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Quant-iT PicoGreen dsDNA Assay Kit a. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. The kit included 20X TE buffer (200 mM Tris-HCl, 20 mM EDTA, pH 7. ZERO BIAS - scores, article reviews, protocol conditions and moreComparison of dsDNA dose-response using the Helixyte Green™ Fluorimetric dsDNA Quantitation Kit *High Sensitivity* (blue) with Quant-iT™ PicoGreen ® dsDNA Assay Kit (red ). Rapidly detect as little as 0. Quantitation Range. Q33130 Introduction The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and. Catalog Number. The final pooled sample was quantified using a Quant-IT PicoGreen dsDNA assay (Thermo Fisher Scientific) and the resulting fragment size distribution was assessed using a Bioanalyzer (Agilent. Catalog Number. The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. 50 pg to 2 µg. Sequencing was performed with a HiSeq Single-End Illumina platform (USA) [Citation 31]. 使用 PicoGreen dsDNA 定量试剂盒和试剂时，即使在存在 ssDNA、RNA 和游离核苷酸的情况下，也可快速检测低至 0. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267The supernatant containing the cytosolic extract was either immediately used or stored at − 80 °C until use. Catalog Number Specifications Unit Size Product Type Quantitation Range Packaging Type Price (EUR) Availability Quantity;The assay was prepared for 184 aliquots of DNA sample and five DNA standards, including one blank. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). Quant-iT™ PicoGreen dsDNA Assay Kit (Life Technologies; Cat. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. Thermo Fisher quant it picogreen dsdna assay kit. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA. dsDNA • Quant-iT PicoGreen dsDNA Assay Kit (Cat. 2023-11. An Agilent 2100 Bioanalyzer (Agilent) was used to compare library quality using a High Sensitivity DNA Kit (Agilent), and the purified library was quantified using a Quant-iT PicoGreen dsDNA assay kit (Invitrogen). 1 mL kit. 製品番号（カタログ番号）： P7581. Touch devices users use touch & swipe gestures. PicoGreen dsDNA quantitation reagents are. Materials: 1. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Mit den Quant-iT Picogreen dsDNA-Assay-Kits und dsDNA-Reagenzien erzielen Sie präzise und präzise dsDNA-Konzentrationsmessungen über einen breiten dynamischen Bereich. Figure 1. high-range. 2 The input amount of linear dsDNA library is based on the available linear dsDNA (see Table 3). The PG reagent from the Quant-iT TM PicoGreen TM dsDNA Assay Kit (Invitrogen) was prepared immediately before use by making a 1:200 dilution of the PG in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7. Per Well Volume 150 uL/well. High linearity and sensitivity over a broad DNA range. 1X dsDNA assay (%) 100 17. Thermo Fisher Scientific公司的Quant-iT PicoGreen dsDNA 检测试剂盒对DNA更特异，比传统光吸收方法的灵敏度高约 1000倍。 如产品手册所述，此实验在微孔板格式中采用单 一染料浓度的动态范围从250 pg/mL到1000 ng/mL。在这 里，我们证明了使用Molecular Devices公司SpectraMax®Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. Final libraries were quantified using a Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) on a SpectraMax iD3 (Molecular Devices). Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™ Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Triplicate 10 µL samples of DNA (), Escherichia coli rRNA or a 1:1 mixture of DNA and RNA were assayed with the Quant-iT dsDNA High-Sensitivity Assay Kit (). 0. 99. You will need 100 ul diluted PicoGreen per well containing 100 ul sample. The tubes were. • Suitable for use in 96- and 384-well plate formats. 86100 stars. Libraries were sequenced on the Illumina NovaSeq. The Quant-iTTM dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. 2. For Research Use Only. Dynamic range and sensitivity of the Quant-iTTM PicoGreen® dsDNA assay. 2023. coli rRNA (Δ), or a 1:1 mixture of DNA and RNA (∎) were assayed in the Quant-iTTM dsDNA HS assay. DNA standards (λ DNA) and DNA samples were stained using a Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Eugene, OR, USA). 0. It is specific for dsDNA in the presence of equimolar amounts of RNA. Q33130). QuantiFluor® ONE dye sensitivity range is 0. An aliquot (1. a) 16. Perform dsDNA quantification easily and quickly with Quant-iT dsDNA assay kits. The assay was prepared for 184 aliquots of DNA sample and five DNA standards, including one blank. 2023. Description. (The same tips can be used. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. High tolerance for contaminants. Quant-iT PicoGreen dsDNA Assay Kit (1mL) Quant-iT PicoGreen dsDNA Reagent (1mL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P7589 P7581: Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen. 3ng/mL，DNAThe fragment size of the amplicon was assessed using agarose gel electrophoresis and quantified by Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific). Quant-IT dsDNA Assay Kit, high sensitivity Quant-iT dsDNA Assay Kit, broad range Quant-iT 1X dsDNA high sensitivity; Target: dsDNA: dsDNA: dsDNA: Quantitation range: 0. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 0 fluorometer according to the manufacturer’s protocols; a sample volume of 1 μl was. This is Standard 1 which will have a final concentration of 1 ug/mL. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Rapidly detect as little as 0. Silke Angersbach, Ron Earp, Mark Gröne, Franka Maurer, BMG LABTECH, 12/2014. The assays are many times more sensitive than conventional UV absorbance and can be optimized for different concentration ranges. For running these libraries on the MiSeq and HiSeq, please make sure you read the supplementary methods of Caporaso et al. For ds DNA can be used kits below: Quant-iT™ PicoGreen ® dsDNA Assay Kit (Invitrogen P7589); Qubit® dsDNA Broad Range Assay Kit (Invitrogen Q32850);. Quant-iT™ PicoGreen® dsDNA Assay KitLife TechnologiesCatalog #P7589 protocols. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Add µL of the Quant-iT™ PicoGreen® dsDNA Reagent (detailed in Section ) to each sample. Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo Fisher Scientific). ZERO BIAS . 1. Material Amount Concentration Storage Stability Quant-iT™ PicoGreen® dsDNA reagent (Component A) 1 mL in 1 vial (P7589) or in10 vials of 100 μL each (P11496) Solution in DMSO 2–6ºC Desiccate Protect from light • • • When stored as directed, product stable. Picogreenアッセイは、ほとんどのマイクロプレートリーダーおよび蛍光光度計に. Note: Allow the Quant-iT ™ RiboGreen RNA Reagent to warm to room temperature before. 9, 10 The Pico-Green assay is sensitive to 25 pg dsDNA/mL, which is 400-fold more sensitive than Hoechst 33258 dye. Quant-iT ™ PicoGreen ™ dsDNA Reagent 1 mL reagent 10 x 100 µL P7581 P11495 TE Buffer (20X), RNase-free 100 mL T11493. dsDNA standards were incubated in cuvettes and measured using a Varian Cary Eclipse. The Quant-iT™ dsDNA BR assay can be adapted for use in cuvettes or 384-well microplates. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. Unit Size. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted RNA standards. RCA products may be directly quantified using the Quant-iT® PicoGreen® dsDNA Assay Kit or with a Qubit® Fluorometer after dilution to the appropriate detection range. 25 pg/uL 的 dsDNA。 该定量试剂盒在三个数量级内呈线性，几乎无序列依赖性，可让您准确测定多种来源的 DNA，包括基因组 DNA、病毒 DNA、小提 DNA 或 PCR 扩. The superior sensitivity and performance of the Modulus™ Microplate Fluorometer allows for dsDNA detection as low as 250ng/mL. Module in combination with PicoGreen® dye allows the direct quantitation of dsDNA in as little as 100 µL total volume. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Picogreen assays are compatible with most microplate readers and fluorometers. Thermo Fisher quant it picogreen dsdna quantitation assay kit. 20 and 2. 2– 100 ng Quant-iT 1X dsDNA HS Assay Kit – Quant-iT PicoGreen Reagent 1–200 ng Quant-iT OliGreen ssDNA Reagent and Assay Kit. • Specific for dsDNA in the presence of RNA. 4 mL of prewarmed to 37°C, TE Buffer Cat# D001 or other qualified TE buffer. The assay is linear over three orders of magnitude and has little sequence dependence. This protocol is a simplified and condensed version of. 20pg/µl for Quant-iT PicoGreen® (1µl of sample in a 200µl assay volume). 50 pg to 2 µg. 使用 Quant-iT Picogreen dsDNA 定量试剂盒和 dsDNA 试剂在较宽的动态范围内实现精准的 dsDNA 浓度测定。. PicoGreen is widely used in molecular biological procedures such as DNA fragment purification for subcloning, cDNA synthesis for library production as well as primer assays. Briefly, 2 µL of sample was diluted in 498 µL TE. Table 2 summarizes results of the. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267DNA concentration was measured using a spectrometer with the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific). Quant-iT™ PicoGreen ® dsDNA Kit (Invitrogen, #P11496). For other nucleic acid methods (RiboGreen, OliGreen), quantified in thePicoGreen reagent is diluted in dimethylsulfoxide (DMSO) which solidifies at refrigerator temperatures. 3 Q33120 39. assay. Quant-iT PicoGreen dsDNA kit by Thermo Scientific Catalog #: P7589 2Prepare 1XTE buffer from 20X stock solution using nuclease-free water: will need 200 μl/well (for. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. 3. Quant-iT dsDNA Assay Kit, broad range Quant-iT 1X dsDNA high sensitivity; Target. Add 5uL of PicoGreen reagent for every 1mL of buffer. Fluorescence was measured at 485/530 nm and plotted versus the mass of nucleic acid for the DNA alone,. Highly selective for dsDNA over ssDNA or RNA. 005% Triton X-100, Tween-20, or Brij. 2. 04 corresponds to 2 μ g/mL dsDNA solution. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile,Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo Fisher Scientific). Rapidly detect as little as 0. Highly selective for dsDNA over ssDNA or RNA. Qubit™ dsDNA Quantification Assay Kits. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. The user guide provides detailed information on the product description, component concentration, storage, assay protocol, and safety data sheets of the Quant-iTTM PicoGreenTM dsDNA Reagent and Kit. The original sequencing data. Product Type. 蛍光を用いたDNAの定量法の一つとしてInvitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kit が利用されています。. Quant-iT ™ PicoGreen ™ dsDNA Assay Kit 1 mL assay kitQuant-iT™ PicoGreen® dsDNA Reagent and Kits | 4 Experimental Protoco l DNA Standard Curve 1. Quantitation using Quant-iT Assay Kits. SYPRO and Coomassie Fluor luminescent and fluorescent protein gel stains—Table 9. This reagent is also included with the Quant-iT RiboGreen RNA Assay Kit and comes complete with buffer and RNA. The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. Quant-iT PicoGreen dsDNA Assay Kit. The Quant-iT™ dsDNA High-Sensitivity Kit is designed for assaying samples containing 0. This was possible since, due to the low height of the light ray of only 8. Description. Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. Total amount of 1X TE per assay will be 200 μl per well which includes the amount of TE used to dilute the PicoGreen reagent. Using this data as a starting point, the optimal conditions (e. Using 20x TE buffer make 1x solution. The use of a serial dilution of dsDNA standards whileThe quantity of ChIP DNA should first be determined with the Quant-iT™ PicoGreen dsDNA Reagent Kit (Invitrogen). dsDNA assays. The AccuBlue® Broad Range RNA Quantitation assay has a linear range of 5-1000 ng RNA in a fluorescent plate reader, a wider range than similar assays. Q33130) Metabolic (cholesterol) • Amplex Red Cholesterol Assay Kit (Cat. Qubit RNA XR Standard can be used with either Qubit RNA XR Assay. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. 実験してみた – PicoGreenをプレートリーダーで使用してみた. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. Catalog Number. 2–100 ng Quant-iT dsDNA HS Assay Kit 0. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. PicoGreen dsDNA quantitation reagents are. 2K. 50 pg to 2 µg. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Reagent Search & Data Services Menu Reagent Search. Libraries were quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Life Technologies) and the Kapa Illumina GA with Revised Primers-SYBR Fast Universal kit (Kapa Biosystems). • Specific for dsDNA in the presence of RNA. 微孔板读数仪. Prediluted standards provided with the Quant-iT assay were used to generate a standard curve. Quant-iT PicoGreen dsDNA Assay Kit and Reagent (Ex/Em = 502/523 nm) dsDNA: 0. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). The AccuBlue® High Sensitivity dsDNA Quantitation Kit offers sensitive and selective detection of purified dsDNA samples with a linear detection range from 0. Revised: 10–June–2008 | MP 07581 Table 1. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. Further to the Quant-iT dsAssay kit (PicoGreen®), the Qubit dsDNA HS Assay kit offers an additional option for quantify-ing dsDNA samples of low concentration on the BioSpec-trometer fluorescence with high specificity and sensitivity. Picogreen assays are compatible with most microplate readers and fluorometers. Picogreen assays are compatible with most microplate readers and fluorometers. DNA standards ranging from 1000 ng/mL to 50 pg/mL were assayed using the Quant-iT PicoGreen dsDNA Assay and SpectraMax M5. dsDNA Assay Kit: Invitrogen Quant-iT PicoGreen dsDNA Assay Kits and dsDNA Reagents, spectrophotometer, qPCR Quantification Kit: Roche KAPA Library Quantification Kits — Complete Kit (Universal), and qPCR Plate Reader: LightCycler 480 Instrument II. lambda DNA standard 100ng/uL (in kit) PicoGreen reagent (in kit) 20x TE buffer (in kit) HPLC water; PicoGreen Prep. Quant-iT PicoGreen Reagent Final DNA Concentration in Quant-iT PicoGreen Assay 0 1000 25 ng/mL 900 100 1000 2. As little as 2. The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. Within two years of follow-up, the primary endpoint defined as nonfatal myocardial infarction, stroke or transient ischemic attack, cardiovascular death, and >80% target vessel restenosis occurred in 34 patients (43%). The fluorescence intensity of PicoGreen was measured to determine the. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). 10 ng/mkl – 10000 ng/ml. 10 x 100 µL. 参考資料. The Quant-iT RNA Reagent and Assay Kit makes RNA quantification easy and accurate. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. in the assay in order to reduce waste and still achieve comparable results. The PicoGreen dsDNA quantitation assay can be used when selectively detecting as little as 25 pg/ml of dsDNA with free nucleotides, RNA and ssDNA present, having minimal impact on the intensity of. Quantification of cfDNA was performed using a TECAN Safire plate reader (96-well format) in combination with the Quant-iT ™ PicoGreen ™ dsDNA Assay Kit (Molecular Probes, Invitrogen; cat #P11496, lot #1911829) according to the manufacturer’s instructions, with slight modifications. USA) and quantity was measured using Qubit 2. Rapidly detect as little as 0. 2. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. This protocol is a simplified and c. Technical Support Customer Service. Description. 50 ul PicoGreen Mix by swirling bottle. Rapidly detect as little as 0. Three kits covering a wide range of dsDNA concentrations. Components of the Quant-iT™ Picogreen™ dsDNA Assay Kit, including the Picogreen reagent and 100 µg/mL λDNA standard stock (Thermo Fisher Scientific), were utilized. 2. Katalognummer: P11496. Picogreen assays are compatible with most microplate readers and fluorometers. Spectrofluorometry was performed based on guidelines from ThermoFisher Scientific Inc. Figure 8. If you would like to use this kit with the Qubit® fluorometer, we have includedQuant-iT ™ PicoGreen ™ dsDNA Reagent 1 mL reagent 10 x 100 µL P7581 P11495 TE Buffer (20X), RNase-free 100 mL T11493. Thermo Fisher quant it picogreen dsdnaassay kit Quant It Picogreen Dsdnaassay Kit, supplied by Thermo Fisher, used in various techniques. No. 99 to $1999. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. Our Quant-iT™ PicoGreen® dsDNA reagent enables researchers to quantitate as little as 25 pg/mL of dsDNA (50 pg dsDNA in a 2 mL assay volume) with a standard spectro fluoro- meter and. PicoGreen dsDNA quantitation reagents are. Product Description. 5 ng/µL. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. Replicates of four were added to a 96-well plate using 2 μL volumes for rows A through D (beige) and 10 μL volumes for rows E through H (blue). Using the Quant-iTTM PicoGreen® dsDNA reagent and the recommended assay protocol, researchers can quantitate dsDNA in the presence of equi molar concentrations of ssDNA and RNA with minimal effect on the quantitation results. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Quant-iT PicoGreen dsDNA Assay Kit 를 사용하면, ssDNA 와 RNA 그리고 free nucleotide 가 혼재되어 있는 시료에서도 dsDNA 만을 선택적으로 측정할 수 있으며, 25 pg /ml 의 매우 적은 양까지도 검출할 수 있습니다. Selection guide for the Qubit and Quant-iT Assay Kits—Table 8. Optimized for fluorescence microplate readers. For duplicate-well droplet. low-range. assay or add 5 µL Quant-iT ™ RiboGreen ™ RNA Reagent to 9. 326,933 327K. Helixyte™ Green exhibits large fluorescence enhancement upon binding to dsDNA. The Quant-iT™ Picogreen® double stranded DNA reagent is added and mixed with both the samples and references through a controlled aspiration and dispense. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Rapidly detect as little as 0. Picogreen assay kit (Invitrogen, P7589) a. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Microplate reader setup for a Quant-iT 1X dsDNA HS Assay. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™Quant-iT PicoGreen dsDNA assay for nucleic acid quantification. P11495. For a standard. Quant-iT ™ PicoGreen ™ dsDNA Assay Kit 1 mL assay kitAchieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. Vortex solution briefly to mix well and centrifuge for 5 sec to bring liquid to bottom of tube; then dispense for use in the assay. Quant It Picogreen Dsdna Quantitation Assay Kit, supplied by Thermo Fisher, used in various techniques. . (Left) The Quant-iT dsDNA Assay Kit, High Sensitivity has a linear detection range of 0. 2 to 100 ng) Assay Kit were used with a Qubit 3. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Generation of a standard curve using dsDNA-Standards Preparation of the measurement solution was based on the user manual of the Quant-iT, PicoGreen dsDNA kit. The Quant-iT dsDNA Broad-Range Kit is designed for assaying samples containing 2–1000 ng of DNA. Quantitation Range. The kit contains: • PicoGreen dsDNA quantitation reagent, 1 ml, stock solution in DMSO • 20 x TE buffer, 25 ml, 200 mM製品番号（カタログ番号）： P11495. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA quantitation, measuring dsDNA in complex mixtures, and viral DNA quantitation. DNA was normalized to 2 concentrations, 100+/-20µg/mL and 20+/-5µg/mL. Picogreen assays are compatible with most microplate readers and fluorometers. Materials: 1. . using a PicoGreen assay by the company life technologies (Quant-iT™ PicoGreen dsDNA Reagent and Kits). (Left) The fluorescence signal from binding of the Quant-iT PicoGreen dsDNA Reagent to dsDNA is linear over at least four orders of magnitude; the inset shows the results obtained using 0–750 pg/ml calf thymus DNA. 1 mL kit. Rapidly detect as little as 0. The DNA standard was Lambda DNA included in the Quant-iT PicoGreen dsDNA Assay Kit (ThermoFisher). Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 19. The stock DNA and the corresponding 1:5 diluted DNA derived from FFPE tissue was quantified by using Quant-iT™ PicoGreen dsDNA Assay kit (Thermo Fisher Scientific) as follows: 2 μl of sample DNA were diluted 1:5 vol/vol in 1× TE, containing 1:100 diluted PicoGreen reagent in a final reaction volume of 40 μl per reaction. Sample fluorescence was. • Suitable for use in 96- and 384-well plate formats. The assay is highly selective for double-stranded DNA (dsDNA) over RNA (Figure 1, page 7) and is accurate for initial sample concentrations from 10 pg/µL to 100 ng/µL. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. Picogreen assays are compatible with most microplate readers and fluorometers. Picogreen assays are compatible with most microplate readers and fluorometers. 2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. We use the Quant-iT™ PicoGreen® dsDNAkit for quantification of both extracted and amplified DNA. The libraries were sequenced using the Illumina HiSeq 2500. 反应次数.